matlab runtime r2022b Search Results


puncta colocalization  (Oxford Instruments)
99
Oxford Instruments puncta colocalization
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
Puncta Colocalization, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab runtime r2022b  (MathWorks Inc)
90
MathWorks Inc matlab runtime r2022b
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
Matlab Runtime R2022b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab r2020b  (MathWorks Inc)
90
MathWorks Inc matlab r2020b
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
Matlab R2020b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab r2023b  (MathWorks Inc)
90
MathWorks Inc matlab r2023b
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
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runtime r2021b for linux  (MathWorks Inc)
90
MathWorks Inc runtime r2021b for linux
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
Runtime R2021b For Linux, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab r2018b  (MathWorks Inc)
90
MathWorks Inc matlab r2018b
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
Matlab R2018b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab r2022a  (MathWorks Inc)
90
MathWorks Inc matlab r2022a
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
Matlab R2022a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r2021b deep learning toolbox  (MathWorks Inc)
90
MathWorks Inc r2021b deep learning toolbox
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
R2021b Deep Learning Toolbox, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ls-dyna  (ANSYS inc)
90
ANSYS inc ls-dyna
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
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optimization toolbox compilation requirements  (MathWorks Inc)
96
MathWorks Inc optimization toolbox compilation requirements
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
Optimization Toolbox Compilation Requirements, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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blockchain framework private ethereum test network  (MathWorks Inc)
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MathWorks Inc blockchain framework private ethereum test network
A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA <t>puncta</t> from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.
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Image Search Results


A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA puncta from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.

Journal: bioRxiv

Article Title: Contrasting synaptic roles of MDGA1 and MDGA2

doi: 10.1101/2023.05.25.542333

Figure Lengend Snippet: A, B, Schematic of the epitope-tagged HA-MDGA1 (A) and Myc-MDGA2 (B). C, D, Representative immunoblot of HA-MDGA1 and Myc-MDGA2 expression in the mouse brain, respectively, from postnatal day 3 to postnatal day 130, using α-tubulin as a loading control. WT P14 sample included for antibody specificity control. E, F, Quantification of HA-MDGA1 / α-tubulin and Myc-MDGA2 / α-tubulin ratios, respectively, normalized to postnatal day 3 values in the KI mice. P14 (peak HA-MDGA1 and Myc-MDGA2 expression) samples from WT mice were used as a control of tag antibody specificity. G, H, Representative immunoblots of HA-MDGA1 and Myc-MDGA2, respectively, using α-tubulin as a loading control, across brain regions. n=3-4 mice/condition for all Western blot analyses. I, Representative HA- MDGA1 immunofluorescence staining in coronal (top panel) and sagittal (bottom panel) slices of HA-MDGA1 mice at P15. J, Representative confocal images of HA staining with DAPI in WT (top) and HA-MDGA1 (bottom) of P20 HA-MDGA1 KI mouse hippocampus. High magnification insets of area CA1 are shown to the right. K, Quantification of HA puncta from high magnification confocal images shown in J (p<0.001, n=8 mice/genotype). Bar graphs represent mean ± SEM. *, p<0.005; **, p<0.01; ***, p<0.001, one-way ANOVA (E, F), unpaired two-tailed Student’s T-Test (K). Scale bars: I, 1 mm. J, 200 µm, insets: 50 µm. “SR”, stratum radiatum; “SP”, stratum pyramidale; “SO”, stratum oriens.

Article Snippet: Images were saved in a “lif.” format, and analysis and quantification of total synaptic puncta and puncta colocalization was performed using Imaris 9.9.1 (Bitplane, South Windsor, CT, USA) and MatLab Runtime R2022b (Mathworks, Natick, MA, USA).

Techniques: Western Blot, Expressing, Control, Immunofluorescence, Staining, Two Tailed Test

A, Low magnification (left) and high magnification (right) representative confocal immunofluorescence images labeling DAPI, HA-MDGA1, and either excitatory (Homer1b/c, top panels) or inhibitory (Nlgn2, bottom panels) postsynaptic marker in P20 HA-MDGA1 mouse hippocampi. B, Quantification of the colocalization of high intensity HA- MDGA1 and synaptic marker puncta (within 5 μm of each other) in the SR. The number of colocalized HA puncta is higher in HA-MDGA1 than WT with Homer1b/c (p <0.0001), but not with Nlgn2 p = 0.2219). In HA-MDGA1 samples, the proportion of HA-MDGA1 puncta colocalized with Homer1b/c is higher than with Nlgn2 (p< 0.0003), whereas in WT samples it is not (p=0.9981). C, D, In the MDGA1-HA/Homer1b/c colocalization experiment, the number of HA puncta was higher in KI samples (p<0.0001, C), but the number of Homer1b/c was not different (p = 0.7481, D). E, F, in the HA-MDGA1/Nlgn2 colocalization experiment, the number of HA puncta was higher in KI samples (p=0.0004, E), but the number of Nlgn2 was not (p = 0.4332, F). G, Low magnification (left) and high magnification (right) representative confocal immunofluorescence images labeling DAPI, HA-MDGA1, and either excitatory (vGluT1, top panels) or inhibitory (vGAT, bottom panels) presynaptic marker in P20 HA-MDGA1 mouse hippocampi. H, Quantification of the colocalization of high intensity HA-MDGA1 and synaptic marker puncta (within 5 μm of each other) in the SR. The number of colocalized HA puncta is higher in HA-MDGA1 than WT (p = 0.0012 with vGluT1, p = 0.049 with vGAT). In neither HA- MDGA1 samples (p = 0.0958) nor in WT samples (p=0.9981) the proportion of HA-MDGA1 puncta colocalized with vGluT1 and vGAT are significantly different. I, J, In the MDGA1- HA/vGluT1 colocalization experiment, the number of HA puncta was higher in KI samples (p<0.0001, I), and the number of vGluT1 was not (p = 0.9033, J). K, L, in the HA- MDGA1/vGAT colocalization experiment, the number of HA puncta was higher in KI samples (p= 0.0029, K), but the number of vGAT was not (p= 0.1157, L). n = 5/8 mice/group. Bar graphs represent mean ± SEM. n.s., non-statistically significant; *, p < 0.05; **, p<0.01; ***, p<0.001, Two-way ANOVA followed Tukey’s post hoc test in B and H and unpaired T-test in C-F and I-L. Scale bars: Left panels: 200 µm. Right panels: 20 µm. “SR”, Stratum Radiatum.

Journal: bioRxiv

Article Title: Contrasting synaptic roles of MDGA1 and MDGA2

doi: 10.1101/2023.05.25.542333

Figure Lengend Snippet: A, Low magnification (left) and high magnification (right) representative confocal immunofluorescence images labeling DAPI, HA-MDGA1, and either excitatory (Homer1b/c, top panels) or inhibitory (Nlgn2, bottom panels) postsynaptic marker in P20 HA-MDGA1 mouse hippocampi. B, Quantification of the colocalization of high intensity HA- MDGA1 and synaptic marker puncta (within 5 μm of each other) in the SR. The number of colocalized HA puncta is higher in HA-MDGA1 than WT with Homer1b/c (p <0.0001), but not with Nlgn2 p = 0.2219). In HA-MDGA1 samples, the proportion of HA-MDGA1 puncta colocalized with Homer1b/c is higher than with Nlgn2 (p< 0.0003), whereas in WT samples it is not (p=0.9981). C, D, In the MDGA1-HA/Homer1b/c colocalization experiment, the number of HA puncta was higher in KI samples (p<0.0001, C), but the number of Homer1b/c was not different (p = 0.7481, D). E, F, in the HA-MDGA1/Nlgn2 colocalization experiment, the number of HA puncta was higher in KI samples (p=0.0004, E), but the number of Nlgn2 was not (p = 0.4332, F). G, Low magnification (left) and high magnification (right) representative confocal immunofluorescence images labeling DAPI, HA-MDGA1, and either excitatory (vGluT1, top panels) or inhibitory (vGAT, bottom panels) presynaptic marker in P20 HA-MDGA1 mouse hippocampi. H, Quantification of the colocalization of high intensity HA-MDGA1 and synaptic marker puncta (within 5 μm of each other) in the SR. The number of colocalized HA puncta is higher in HA-MDGA1 than WT (p = 0.0012 with vGluT1, p = 0.049 with vGAT). In neither HA- MDGA1 samples (p = 0.0958) nor in WT samples (p=0.9981) the proportion of HA-MDGA1 puncta colocalized with vGluT1 and vGAT are significantly different. I, J, In the MDGA1- HA/vGluT1 colocalization experiment, the number of HA puncta was higher in KI samples (p<0.0001, I), and the number of vGluT1 was not (p = 0.9033, J). K, L, in the HA- MDGA1/vGAT colocalization experiment, the number of HA puncta was higher in KI samples (p= 0.0029, K), but the number of vGAT was not (p= 0.1157, L). n = 5/8 mice/group. Bar graphs represent mean ± SEM. n.s., non-statistically significant; *, p < 0.05; **, p<0.01; ***, p<0.001, Two-way ANOVA followed Tukey’s post hoc test in B and H and unpaired T-test in C-F and I-L. Scale bars: Left panels: 200 µm. Right panels: 20 µm. “SR”, Stratum Radiatum.

Article Snippet: Images were saved in a “lif.” format, and analysis and quantification of total synaptic puncta and puncta colocalization was performed using Imaris 9.9.1 (Bitplane, South Windsor, CT, USA) and MatLab Runtime R2022b (Mathworks, Natick, MA, USA).

Techniques: Immunofluorescence, Labeling, Marker